Antibacterial effect of Aqueous Neem (Azadirachta indica) leaf extract, crude neem leaf paste, and Ceftriaxone against Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa
DOI:
https://doi.org/10.18034/mjmbr.v3i1.401Keywords:
Antibacterial effect, crude neem leaf paste (CNLP), Aqueous neeem leaf extract (ANLE), Ceftraxione (antibiotic), Minimum inhibitory concentration (MIC), Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosaAbstract
The study was conducted during the period of July 2004 to June 2005 in the Department of Pharmacology & Therapeutics in collaboration of Department of Microbiology, Mymensingh Medical College, Mymensingh to determine the profile of antibacterial effect of crude neem leaf paste (CNLP), aqueous neem leaf extract (ANLE), and standard antibiotic Ceftriaxone against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. Five separate experiments were done e.g. I) Determination of inhibitory effect of crude neem leaf paste (CNLP) by incorporation into nutrient agar media (NA), against Staphylococcus aureus, Escherichia coli and, Pseudomonas aeruginosa. II) Determination of minimum inhibitory concentration (MIC) of aqueous neem leaf extract (ANLE) against that three test organisms by broth dilution technique, III) Determination of minimum inhibitory concentration (MIC) of standard antibiotic ceftriaxcone against test organisms by broth dilution technique as well as making a comparison with MIC of ANLE and IV) Subculture study of materials from effective CNLP, ANLE, NLEE and Ceftriaxone in nutrient agar medium for confirmation of respective results of different experiments conducted. Results revealed that inhibitory effects were observed against the growth of Staph. aureus, Esch. coli and Ps. aeruginosa at 15%, 20% and 25% respectively of CNLP incorporated into NA media. The broth dilution technique was followed to determine the MICs of ANLE and Ceftriaxone. The MIC of ANLE was 714 μg/ml against S. aureus and that against E. coli and P. aeruginosa was 1428 μg/ml. The MIC of Ceftriaxone was 10μg/ml against S. aureus and that against E. coli and P. aeruginosa was 25 μg/ml. The MIC of Ceftriaxone was the lowest in comparison to MICs of ANLE. The subculture study showed similar results with that of previous experiments in terms of inhibitory effects of CNLP and MICs of ANLE, Ceftriaxone against all of the organisms studied.
Downloads
References
Chaturvedi DD, Chopra KK and Tumane HM. Neem in Ayurveda: An overview. Neem applications in Agriculture, Health care and Environment. A publication of Neem Foundation 67-A, Vithalnagar, Road no. 12 NS. Juhu Scheme, Mumbai-400 049 India. 1998: 57.
Chaurasia SC and Jain PC. Antibacterial Activity of Essential oils of Four Medicinal plants. Indian J. Hospital Pham Nov – Dec 1978:166-168.
Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J. Pharmacol. 2000; 32: S81–S118.
Das BK, Mukherjee SC, Sahu BB, Murijani G. Neem (Azadirachta Indica) extract as an antibacterial agent against fish pathogenic bacteria. Indian J. Exp. Biol.[ serial on line] 1999 Nov; 37(11): 1097–100.
Gani A. Bheshaja Oshudh (Herbal Medicine). 1st ed. Bangla Academy; Dhaka, Bangladesh. March 2002: 1–86.
Gani A. Need and importance of using traditional medicine in current perspective of Bangladesh. Hamdard Samachar Dhaka 1205, Bangladesh. 2003; July vol. 3-4. www.hamdard.bd.com
Kabra KN. and Upadhyay V. Development and Ecological Role of Neem in India. The Neem Foundation, Mumbai. Indian Inst. Pub. Administration, I.P. Estate, Ring road, New Delhi–110 002. 2000: 1-15.
Natarajan V, Venugopal PV, Menon T. Effect of Azadirachta indica (Neem) on the growth pattern of Dermatophytes. Indian Journal of Medical Microbiology 2003; 21 (2): 98 – 101.
Neem Tree Farms Company. Advantages of Neem. [Screens 6] Available at: www.neemtreeframs.com Accessed. January 14, 2005.
Parida MM., Upadhyay C, Pandya G, Jana AM Inhibitory potential of neem (Azadirachta Indica Juss) leaves on dengue virus type-2. replication. J. Ethnopharmacol. 2002 Feb; 79(2): 273-8.
Tewari DN. Monograph on Neem. Published in India by RP Singh, International Book Distributors, 9/3, Rajpur Road, Dehra, Dun-248001. India.1992: 1-157.
Upadhyay SN. Neem as a cost effective solution for population control. Neem applications in Agriculture, Health care and Environment. A publication of Neem Foundation. 67-A, Vithalnagar, Road no. 12 NS, Juhu Scheme, Mumbai-400 049. India. 1998: 58.
-- 0 --
