HPTLC Profiles and Antioxidant Activities from Leaves to Green and Roasted Beans of Coffea Arabica
DOI:
https://doi.org/10.18034/mjmbr.v5i1.446Keywords:
Coffea arabica, leaves, HPTLCAbstract
The health benefits of coffee consumption are a very actual research subject, given the fact that is one of the most popular beverages in the world. The majority of the studies are concentrated to coffee beans (green or roasted) chemical composition (the most important non-volatile compounds investigated being phenols and alkaloids) and pharmacological activity. Green coffee is now in the market in the form of food supplements products. In the present, the chemical composition and bioactivity of the leaves occupy a small place in scientific papers.
This research paper investigate, in terms of caffeine, phenolic compounds composition and antioxidant activities, the differences and similarities between hydroalcoholic extracts of Coffea arabica leaves and green and roasted beans. The extracts profiles, determined by HPTLC technique, shows the major phenolic compounds. Through chromatographic fingerprint, the presence and the amount of caffeine in the extracts were also determined. The total phenolic content (TPC) (Folin Ciocalteu method) expressed as gallic acid equivalents decrease from the leaves to green and roasted coffee beans extracts. The IC50 (concentration of sample required to inhibit 50% of the DPPH free radical) was determined by free radical scavenging activity of Dpph. The IC50 values were TPC concentration-dependent. The obtained results show that in the hydroalcoholic extracts of the leaves are almost the same major phenolic compounds as in the green coffee beans extract. Also, the leaves extract have a higher content in total phenols and a better antioxidant activity comparative to the other samples. Therefore, this paper can be the first step for further investigations of coffee leaves extracts, which might have important health beneficial effects and can be a reliable raw material for food supplements industry.
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References
Adrian J, Frangne R. Synthesis and availability of niacin in roasted coffee. Adv Exp Med Biol. 1991;289:49-59. Review.
Ashihara Hiroshi Coffee in Health and Disease Prevention Chapter 3 – Plant Biochemistry: Trigonelline Biosynthesis in Coffea arabica and Coffea canephora 2015, Pages 19–28).
Ballesteros LF, Cerqueira MA, Teixeira JA, Mussatto SI. Characterization of polysaccharides extracted from spent coffee grounds by alkali pretreatment. Carbohydr Polym. 2015 Aug 20;127:347-54.;
Clarke R.J., Macrae R. , Coffee: Physiology, Springer Science & Business Media, Dec 31, 1988;
Dong W, Tan L, Zhao J, Hu R, Lu M. Characterization of Fatty Acid, Amino Acid and Volatile Compound Compositions and Bioactive Components of Seven Coffee (Coffea robusta) Cultivars Grown in Hainan Province, China. Molecules. 2015 Sep 14;20(9):16687-708;
Duarte, G., Farah, A. Chlorogenic acids and lactones in Brazilian commercial coffee. Proc. 22nd Int. Conf. Coffee Sci. ASIC/Prospero, 224–227. 2009. Trieste, Italy;
Dupas, C. J., Marsset-Baglieri, A. C., Ordonaud, C. S., Ducept, F. M. G. and Maillard, M.-N. (2006), Coffee Antioxidant Properties: Effects of Milk Addition and Processing Conditions. Journal of Food Science, 71: S253–S258;
Farah Adriana, Duarte Giselle Coffee in Health and Disease Prevention, Chapter 87–Bioavailability and Metabolism of Chlorogenic Acids from Coffee 2015, Pages 789–801 )
Farah, A., Donangelo, C. M. Phenolic compounds in coffee. Braz. J. Plant Physiol. 2006, 18, 26–36;
Farah, A. (2012) Coffee Constituents, in Coffee: Emerging Health Effects and Disease Prevention (ed Y.-F. Chu), Wiley-Blackwell, Oxford, UK).
Flament, I., Gautschi, F., Winter, M., Willhalm, B., Stoll, M. Les composants furanniques de l’arˆ ome cafe: quelques aspects chimiques et spectroscopiques. Proc. 3rd Coll. Int. Coffee Sci. ASIC, 197–215.1968. Paris
Folstar P, van der Plas HC, Pilnik W, de Heus JG. Tocopherols in the unsaponifiable matter of coffee bean oil. J Agric Food Chem. 1977 Mar-Apr;25(2):283-5.)
Ochiai R, Sugiura Y, Shioya Y, Otsuka K, Katsuragi Y, Hashiguchi T. Coffee polyphenols improve peripheral endothelial function after glucose loading in healthy male adults. Nutr Res. 2014 Feb;34(2):155-9).,
Preedy Victor R. Caffeine: Chemistry, Analysis, Function and Effects Royal Society of Chemistry, 2012 -
Sanchez-Moreno, C. Larrauri, J.A., Saura-Calixto, F., 1998. A procedure to measure the antiradical efficiency of polyphenols. Journal of Agricultural and Food Chemistry 76, 270-276
Somporn C, Kamtuo A, Theerakulpisut P, Siriamornpun S. Effect of shading on yield, sugar content, phenolic acids and antioxidant property of coffee beans (Coffea Arabica L. cv. Catimor) harvested from north-eastern Thailand. J Sci Food Agric. 2012 Jul;92(9):1956-63.
Tuomilehto J. [Coffee and health]. Duodecim. 2013;129(13):1398-405;
Wagner H., Bladt S., 1996. Plant Drug Analysis, Second Edition, Springer.
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